Compositional editing of extracellular matrices by CRISPR/Cas9 engineering of human mesenchymal stem cell lines
eLife, 2024. Sujeethkumar Prithiviraj, Alejandro Garcia Garcia, Karin Linderfalk, Bai Yiguang, Sonia Ferveur, Ludvig Nilsén Falck, Agatheeswaran Subramaniam, Sofie Mohlin, David Hidalgo, Steven J Dupard, Deepak Bushan Raina, Paul E Bourgine Abstract Tissue engineering strategies predominantly rely on the production of living substitutes,
Sustained delivery of a heterodimer bone morphogenetic protein-2/7 via a collagen hydroxyapatite scaffold accelerates and improves critical femoral defect healing
Acta Biomaterialia., 2023. YangLiu, Manoj Puthia, Eamon J. Sheehy, Ines Ambite, Jitka Petrlova, Sujeethkumar Prithviraj, Maria Wimer Oxborg, Sujeesh Sebastian, Corina Vater, Stefan Zwingenberger, André Struglics, Paul E. Bourgine, Fergal J. O’Brien, Deepak Bushan Raina Abstract Despite the glimmer of hope provided by
Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments
Front. Bioeng. Biotechnol., 2023. Steven J. Dupard, Alejandro Garcia Garcia, Paul E Bourgine. Abstract Faithful modeling of tissues and organs requires the development of systems reflecting their dynamic 3D cellular architecture and organization. Current technologies suffer from a lack of design flexibility and
Engineering human mini-bones for the standardized modeling of healthy hematopoiesis, leukemia, and solid tumor metastasis
Science Translational Medicine, 2022. Ani Grigoryan, Dimitra Zacharaki, Alexander Balhuizen, Christophe RM Côme, Alejandro Garcia Garcia, David Hidalgo Gil, Anne-Katrine Frank, Kristina Aaltonen, Adriana Mañas, Javanshir Esfandyari, Pontus Kjellman, Emelie Englund, Carmen Rodriguez, Wondossen Sime, Ramin Massoumi, Nasim Kalantari, Sujeethkumar Prithiviraj,
Culturing patient-derived malignant hematopoietic stem cells in engineered and fully humanized 3D niches
PNAS, 2021. Andrés García-García, Thibaut Klein, Gordian Born, Morgane Hilpert, Arnaud Scherberich, Claudia Lengerke, Radek C. Skoda, Paul E. Bourgine, Ivan Martin Abstract Human malignant hematopoietic stem and progenitor cells (HSPCs) reside in bone marrow (BM) niches, which remain challenging to explore due to limited in vivo accessibility and constraints with humanized animal models. Several in vitro systems
Manufacturing of Human Tissues as off-the-Shelf Grafts Programmed to Induce Regeneration
Advanced materials, 2021. Pigeot, S., Klein, T., Gullotta, F., Dupard, S. J., Garcia, A., García-García, A., Prithiviraj, S., Lorenzo, P., Filippi, M., Jaquiery, C., Kouba, L., Asnaghi, M. A., Raina, D. B., Dasen, B., Isaksson, H., Önnerfjord, P., Tägil, M., Bondanza, A., Martin, I., Bourgine, P. E. Abstract Design criteria for tissue-engineered materials in regenerative medicine include robust biological effectiveness, off-the-shelf availability,
3D Engineering of Human Hematopoietic Niches in Perfusion Bioreactor
Springer Methods in Molecular Biology, 2021. Dupard S, Bourgine PE. Abstract The hematopoietic microenvironment, also referred to as hematopoietic niche, is a functional three-dimensional (3D) unit of the bone marrow (BM) that planar culture systems cannot recapitulate. Existing limitations of 2D protocols are
Engineering human hematopoietic environments through ossicle and bioreactor technologies exploitation
Experimental Hematology, 2020. Sommerkamp P, Mercier FE, Wilkinson AC, Bonnet D, Bourgine PE. Abstract The bone marrow microenvironment contains cellular niches that maintain the pool of hematopoietic stem and progenitor cells and support hematopoietic maturation. Malignant hematopoietic cells also co-opt normal cellular interactions in order to promote their
Extracellular matrix reinforced bioinks for 3D bioprinting human tissue
Advanced Materials, 2020. De Santis MM, Alsafadi HN, Tas S, Bölükbas DA, Prithiviraj S, Da Silva IAN, Mittendorfer M, Ota C, Stegmayr J, Daoud F, Königshoff M, Swärd K, Wood JA, Tassieri M, Bourgine PE, Lindstedt S, Mohlin S, Wagner DE. Abstract Recent
Orthotopic Bone Formation by Streamlined Engineering and Devitalization of Human Hypertrophic Cartilage
International Journal of Molecular Sciences, 2020. Pigeot S, Bourgine PE, Jaquiery C, Scotti C, Papadimitropoulos A, Todorov A, Epple C, Peretti GM, Martin I. Abstract Most bones of the human body form and heal through endochondral ossification, whereby hypertrophic cartilage (HyC) is formed and